Stomata length is a reliable characteristic for distinguishing infraspecies and ploidy levels of Opuntia mesacantha (Cactaceae)

Autores/as

  • Paul Adanick Department of Agriculture, Eastern Kentucky University
  • Taly Dawn Drezner Department of Geography, York University
  • A. Dean Stock

DOI:

https://doi.org/10.17348/jbrit.v12.i1.925

Palabras clave:

cactus, field techniques, polyploidy, prickly pear

Resumen

Opuntia mesacantha incluye dos subespecies, O. mesacantha subsp. mesacantha (tetraploide) y O. mesacantha subsp. lata (diploide), que son difíciles de distinguir en el campo. Nosotros señalamos (1) la longitud de los estomas es efectiva para distinguir las dos subespecies, y (2) esto puede asegurarse visualmente, es efectivo y no-destructivo, usando un microscopio con un retículo (ocular). Comparamos nuestros resultados con los imagen digital y recuentos cromosomáticos en mitosis ápices radiculares para confirmar que nuestro sistema es efectivo para estos taxa.

Citas

Baker, M.A. 2016. Morphological and cytological analyses in Cylindropuntia (Cactaceae): The taxonomic circumscription of C. echinocarpa, C. multigeniculata, and C. whipplei. J. Bot. Res. Inst. Texas 10:325–343.

Beck, S.L., R.W. Dunlop, & A. Fossey. 2003. Stomatal length and frequency as a measure of ploidy level in black wattle, Acacia mearnsii (de Wild). Bot. J. Linn. Soc. 141:177–181.

Casson, S. & J.E. Gray. 2008. Influence of environmental factors on stomatal development. New Phytol. 178:9–23.

Conde, L.F. 1975. Anatomical comparisons of five species of Opuntia (Cactaceae). Ann. Missouri Bot. Gard. 62:425–473.

Conger, A.D & L.M. Fairchield. 1953. A quick freeze method for making smear slides permanent. Stain Tech 28:281–283.

Drezner, T.D. 2017a. North and south: Morphological variability in the endangered Opuntia cespitosa in Canada and variation with environmental conditions. Castanea 82:8–23.

Drezner, T.D. 2017b. Shade, reproductive effort and growth of the endangered native cactus, Opuntia humifusa, in Point Pelee National Park, Canada. J. Torrey Bot. Soc. 144:179–190.

Herrera-Martinez ,V., L. Rios-Hernandez, C. Garciduenas-Pina, A. Lara-Ibarra, A. Adabache-Ortiz, R.E. Soria-Guerra, E. Perez-Molphe-Balch, & J.F. Morales-Dominguez. 2015. Effect of culture conditions on stomatal density and stomatal index in four cactus species. Haseltonia 20:43–50.

Hilu, K.W. & J.L. Randall. 1984. Convenient method for studying grass leaf epidermis. Taxon 33:413–415.

Joachimiak, A. & A. Grabowska-Joachimiak. Stomatal cell length and ploidy level in four taxa belonging to the Phleum sect. Phleum. Acta Biol. Cracoviensia 42:103–107.

Majure, L.C., W.S. Judd, P.S. Soltis, & D.E. Soltis. 2012. Cytogeography of the Humifusa clade of Opuntia s.s. Mill. 1754 (Cactaceae: Opuntioideae): Correlations with geographic distributions and morphological differentiation of a polyploid complex. Comp. Cytogenet. 6:53–77.

Majure, L.C. & E. Ribbens. 2012. Chromosome counts of Opuntia (Cactaceae), prickly pear cacti, in the Midwestern United States and environmental factors restricting the distribution of Opuntia fragilis. Haseltonia 17:58–65.

Majure, L.C., W.S. Judd, P.S. Soltis, & E. Soltis. 2017. Taxonomic revision of the Opuntia humifusa complex (Opuntieae: Cactaceae) of the eastern United States. Phytotaxa 290:1–65.

Masterson, J. 1994. Stomatal size in fossil plants: Evidence for polyploidy in majority of angiosperms. Science 264:421–424.

Mishra, M.K. 1997. Stomatal characteristics at different ploidy levels in Coffea L. Ann. Bot. 80:689–692.

Nobel, P.S. 1982. Orientations of terminal cladodes of platyopuntias. Bot. Gaz. 143:219–224.

Parfitt, B.D. 1980. Origin of Opuntia curvospina (Cactaceae). Syst. Bot. 5:408–418.

Przywara, L., K.K. Pandey, & P.M. Sanders. 1988. Length of stomata as an indicator of ploidy level in Actinidia deliciosa. New Zealand J. Bot. 26:179–182.

Shrestha, S.L. & W.H. Kang. 2016. Stomata length and density as an indicator of ploidy level in sweet pepper (Capsicum annuum L.). Res. J. Recent Sci. 5:4–10.

Stock, A.D., D.B. Burnham, & T.C. Hsu. 1972. Giemsa banding of meiotic chromosomes with a description of a procedure for cytological preparations from solid tissues. Cytogenetics 11:534.

Tanaka, R. & H. Kamemoto. 1984. Chromosomes in Orchids; counting and numbers. In: J. Arditti, ed. Orchid biology; reviews and perspectives III. Cornell University Press, Ithaca, New York, U.S.A. Pp. 324–410.

Zheng, Y., M. Xu, R. Hou, R. Shen, S. Qiu, & Z. Ouyang. 2013 Effects of experimental warming on stomatal traits in leaves of maize (Zea may L.). Ecol. Evol. 3:3095–3111.

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Publicado

2018-07-20

Cómo citar

Adanick, P., Drezner, T. D., & Stock, A. D. (2018). Stomata length is a reliable characteristic for distinguishing infraspecies and ploidy levels of Opuntia mesacantha (Cactaceae). Journal of the Botanical Research Institute of Texas, 12(1), 141–147. https://doi.org/10.17348/jbrit.v12.i1.925